Sialyl-Lewis X
 | |
| Identifiers | |
|---|---|
CAS Number |
|
3D model (JSmol) |
|
ChEMBL |
|
ChemSpider |
|
MeSH | sialyl+Lewis+X |
PubChem CID |
|
InChI
| |
SMILES
| |
| Properties | |
Chemical formula | C31H52N2O23 |
Molar mass | 820.744 g/mol |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
 Y verify (what is  Y N ?) | |
Infobox references | |
Sialyl LewisX, also known as sialyl LeX or SLeX, is a tetrasaccharide carbohydrate with the sequence Neu5Acα2-3Galβ1-4[Fucα1-3]GlcNAcβ- that is usually attached to O-glycans on the surface of cells. It is known to play a vital role in cell-to-cell recognition processes. It is also the means by which an egg attracts sperm; first, to stick to it, then bond with it and eventually form a zygote. The discovery of the essential role that this tetrasaccharide plays in the fertilization process was reported in August 2011.[1]
Sialyl Lewis X is also one of the most important blood group antigens and is displayed on the terminus of glycolipids that are present on the cell surface. The sialyl Lewis X determinant, E-selectin ligand carbohydrate structure, is constitutively expressed on granulocytes and monocytes and mediates inflammatory extravasation of these cells. Resting T- and B-lymphocytes lack its expression and are induced to strongly express sialyl Lewis X upon activation. The sialyl Lewis X determinant is expressed preferentially on activated Th1 cells but not on Th2 cells.
Contents
1 History
2 Clinical significance
3 Function
3.1 Leukocyte homing and cancer metastasis
3.2 Human fertilization
4 See also
5 References
6 Sources
History
The term Lewis in its name comes from the name of a family of people who suffered from a red blood cell incompatibility. The studies done on these individuals’ red blood cells led to the discovery of sialyl Lewis x. Sialyl Lewis x is a very important red blood cell antigen present on the glycolipids on the plasma membrane of the cell.
Clinical significance
Defective synthesis of the sialyl Lewis X antigen results in immunodeficiency (leukocyte adhesion deficiency type 2). Defective synthesis can be caused by the loss of fucosyltransferase, impairing the glycosylation of the glycosphingolipid.
Sialyl Lewis x is being used in studies to fight tumors and cancer cell growth. It has been shown that there is frequent overexpression of sialyl Lewis x on cancer cells and is found on both N-glycan and O-glycans. Sialyl Lewis x is being researched with CD markers to find new ways to create biosensors for cancer cells. Also, it is being used in new ways to target cancer cells specifically for cancer treatment.
It is also being used to achieve greater rates of fertilization of eggs in women by coating the eggs with sialyl Lewis x. It plays a key role in the inflammatory response and may be used to increase the leukocyte response to infections.
Sialyl Lewis x is an inflammation-associated antigen on liver cells. It becomes over expressed on diseased liver cells and can be used as a way to detect liver disease in a patient.
Sialyl Lewis x is also being researched for detection and treatment of immune disorders because of its presence on leukocytes. There is congenital disorder where there is an inclination to recurring severe infections. This stems from an absence of sialyl Lewis x attached to E-selectin ligands on their neutrophils.
Function
Leukocyte homing and cancer metastasis
Sialyl-Lewisx is important in leukocyte tethering and rolling. Leukocytes move through the blood stream and then tether themselves to the endothelial wall and roll along the endothelial tissue to determine if they want to leave the bloodstream to get to necessary tissue.
Sialyl-Lewisx is a necessary partner for the three selectins that bind the leukocyte and endothelial cells. When sialyl-Lewisx is part of an O-glycan and attached to CD34 it can then bind to L-selectin. For the binding to L-selectin to occur sialyl-Lewisx must undergo sulfation. For sialyl-Lewisx to bind to P-selectin, an O-linked glycan near the N-terminus of P-Selectin Glycoprotein Ligand-1 (PSGL-1) is modified with sialyl-Lewisx and in combination with nearby tyrosine residues modified with sulfate, forms the binding contact for P-selectin. For sialyl-Lewisx to bind to E-selectin it can be part of either an N-linked or O-linked glycan attached to cell surface glycoproteins such as PSGL-1, CD43 or CD44. This sialyl-Lewisx mediated binding to selectins allows circulating leukocytes to stick to and roll along endothelial cells lining blood vessels thereby enabling the leukocytes to accumulate at a site of vascular inflammation.
Sialyl-Lewisx also plays a critical role in cancer metastasis, facilitating the extravasation of cancer cells out of the bloodstream while they are moving through the body.
Human fertilization
Sialyl-Lewisx allows a sperm cell to recognize and fertilize an egg cell. For fertilization to occur, human sperm must bind to the zona pellucida (ZP), the translucent matrix covering the human egg composed of four glycoproteins ZP1, 2, 3, and 4, and transit through the matrix in order to fuse with the oocyte.[2] Human ZP is coated with highly dense N- and O-glycans that are terminated with the sialyl-Lewisx sequence.[3] The hemizona assay, which assesses sperm-ZP binding by counting the number of sperm bound to hemispheres of bisected nonliving human eggs in vitro, revealed that as little as 0.5 mM sialyl-Lewisx inhibits sperm-ZP binding by 63%.[3] Furthermore, adding purified and solubilized ZP3 or ZP4 from the human oocyte dose-dependently inhibits sperm-ZP binding in the hemizona assay.[4] Such evidence suggest that the early steps of human sperm-egg binding may be mediated by lectins for sialyl-Lewisx present on human sperm.
See also
CD15 (Lewis X)- Sialyl lewis a
References
^ Pang, Poh-Choo; Chiu, Lee, Chang; et al. (18 August 2011). "Human Sperm Binding Is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida". Science. 333 (6050): 1761–1764. doi:10.1126/science.1207438. PMID 21852454. Retrieved 23 October 2011.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link) .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output q{quotes:"""""""'""'"}.mw-parser-output code.cs1-code{color:inherit;background:inherit;border:inherit;padding:inherit}.mw-parser-output .cs1-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .cs1-lock-limited a,.mw-parser-output .cs1-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .cs1-lock-subscription a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Lock-red-alt-2.svg/9px-Lock-red-alt-2.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration{color:#555}.mw-parser-output .cs1-subscription span,.mw-parser-output .cs1-registration span{border-bottom:1px dotted;cursor:help}.mw-parser-output .cs1-hidden-error{display:none;font-size:100%}.mw-parser-output .cs1-visible-error{font-size:100%}.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration,.mw-parser-output .cs1-format{font-size:95%}.mw-parser-output .cs1-kern-left,.mw-parser-output .cs1-kern-wl-left{padding-left:0.2em}.mw-parser-output .cs1-kern-right,.mw-parser-output .cs1-kern-wl-right{padding-right:0.2em}
^ Clark, G. F. (2013). "The role of carbohydrate recognition during human sperm-egg binding". Human Reproduction. 28 (3): 566–577. doi:10.1093/humrep/des447. PMID 23315069.
^ ab Pang, P. -C.; Chiu, P. C. N.; Lee, C. -L.; Chang, L. -Y.; Panico, M.; Morris, H. R.; Haslam, S. M.; Khoo, K. -H.; Clark, G. F.; Yeung, W. S. B.; Dell, A. (2011). "Human Sperm Binding is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida". Science. 333 (6050): 1761–1764. doi:10.1126/science.1207438. PMID 21852454.
^ Chiu, P. C. N.; Wong, B. S. T.; Chung, M. -K.; Lam, K. K. W.; Pang, R. T. K.; Lee, K. -F.; Sumitro, S. B.; Gupta, S. K.; Yeung, W. S. B. (2008). "Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa". Biology of Reproduction. 79 (5): 869–877. doi:10.1095/biolreprod.108.069344. PMID 18667750.
- Etzioni, Amos; Frydman, Moshe; Pollack, Shimon; Avidor, Israeli; Phillips, M. Laurie; Paulson, James C.; Gershoni-Baruch, Ruth; Recurrent Severe Infections Caused by a Novel Leukocyte Adhesion Deficiency. Journal Article (1992) New England Journal of Medicine 1789-1792; 327. http://www.nejm.org/doi/full/10.1056/NEJM199212173272505
Sarangapani, Krishna K.; Qian, Jin; Chen, Wei; Zarnitsyna, Veronika I.; Mehta, Padmaja; Yago, Tadayuki; McEver, Rodger P.; Zhu, Cheng (2011). "Regulation of Catch Bonds by Rate of Force Application". J. Biol. Chem. 286 (37): 32749–32761. doi:10.1074/jbc.M111.240044. PMC 3173187. PMID 21775439.
Li-Hua Pan; Kohei Yamauchi; Takashi Sawai; Toshihide Nakadate; Yuki Kojima; Naofumi Takahashi; Keisuke Adachi; Akihiko Kameyama & Hiroshi Inoue (May 2000). "Inhibition of binding of E- and P-selectin to sialyl-Lewis X molecule suppresses the inflammatory response in hypersensitivity pneumonitis in mice". Am J Respir Crit Care Med. 161 (5): 1689–97. doi:10.1164/ajrccm.161.5.9812016. PMID 10806176.
Pang, P. -C.; Chiu, P. C. N.; Lee, C. -L.; Chang, L. -Y.; Panico, M.; Morris, H. R.; Haslam, S. M.; Khoo, K. -H.; Clark, G. F.; Yeung, W. S. B.; Dell, A. (2011). "Human Sperm Binding is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida". Science. 333 (6050): 1761–1764. doi:10.1126/science.1207438. PMID 21852454.
Sources
- Stephen J Isles, Molecule of the Month: Sialyl Lewis X
Chen G, Osada H, Santamaria-Babi L, Kannagi R; Osada; Santamaria-Babi; Kannagi (2006). "Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes". Proc Natl Acad Sci USA. 103 (45): 16894–9. doi:10.1073/pnas.0607926103. PMC 1629005. PMID 17075044.CS1 maint: Multiple names: authors list (link)
- Essentials of Glycobiology 3rd Edition, Chapter 14: "Structures Common to Different Glycans" https://www.ncbi.nlm.nih.gov/books/NBK453042/#_Ch14_s2_
